Microbial Biosynthesis of Antibacterial Chrysoeriol in Recombinant Escherichia coli and Bioactivity Assessment

Advances and challenges in recombinant protein expression in Escherichia coli

Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli

Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria. Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC sy...

Recombinant strains of Escherichia coli for L-aspartic acid biosynthesis.

The aspartase overproducing mutant B-715 was used as a donor of the aspartase gene for further construction of the aspartase-hyperproducing strains by molecular cloning. In preliminary experiments activity of transformants and their efficiency in L-aspartic acid biosynthesis were compared. The conditions for recombinant strain multiplication, biomass activation and L-aspartic acid biosynthesis ...

Expression, purification and osteogenic bioactivity of recombinant human BMP-2 produced by Escherichia coli

INTRODUCTION: Among BMP family, BMP-2 is the best characterized molecule and it has the strongest bone-inducing activity. Previously, most rhBMPs have been purified mainly from mammals, like Chinese hamster ovary (CHO) cells. Problems associated with clinical application of CHO-derived recombinant human bone morphogenetic protein are post-translational problems, its low yields and high cost due...

Enzyme Biosynthesis in Escherichia Coli

Escherichia coli B synthesized beta-galactosidase and an enzyme system for D-xylose when exposed to lactose and xylose respectively in nitrogen-free media. The amount of beta-galactosidase formed in the absence of external nitrogen depended upon the nature of the medium in which the cells had originally been grown. Half as much of this enzyme was synthesized without exogenous nitrogen by cells ...